DAta SElf-Recalibration and Mixture Mass Fingerprint Searching (DASER-MMF) to Enhance Proteomics Identifications from Complex Mixtures.

Journal of the American Chemical Society (2008 19:1914-1925)

Danell, R., Ouvry-Patat, S.A., Scarlett, C.O., Speir, J.P. and Borchers C.H.

A novel algorithm based on Data Self-Recalibration and a subsequent Mixture Mass Fingerprint search (DASER-MMF) has been developed to improve the performance of protein identification from online 1D and 2D-LC-MS/MS experiments conducted on high-resolution mass spectrometers. Recalibration of 40% to 75% of the MS spectra in a human serum dataset is demonstrated with average errors of 0.3 +/- 0.3 ppm, regardless of the original calibration quality. With simple protein mixtures, the MMF search identifies new proteins not found in the MS/MS based search and increases the sequence coverage for identified proteins by six times. The high mass accuracy allows proteins to be identified with as little as three peptide mass hits. When applied to very complex samples, the MMF search shows less dramatic performance improvements. However, refinements such as additional discriminating factors utilized within the search space provide significant gains in protein identification ability and indicate that further enhancements are possible in this realm.

The software algorithms highlighted in this publication will be made available to the public.
For more information, please contact Dr. Christoph Borchers: